The EU has ringfenced 35,000t of its hormone-free beef quota for US imports, phased over a seven-year period.
The remaining 10,000t of quota will be left available for other exporters.
"With the successful outcome of the negotiations, the Commission has delivered on a very important issue with a major trade partner with which we are engaged in broader trade talk,” European Commissioner for Agriculture Phil Hogan said.
“I also want to reiterate that the agreement will not change the overall volume, quality or safety of the beef imported into the EU, which will remain in compliance with the high European standards."
Following the conclusion the negotiations, the Commission will submit legal proposals for the Council to authorise its signature and to conclude the agreement with the United States in the coming months, after seeking the consent of the European Parliament.
EU to discuss import of hormone-free beef with US
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